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Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 100-104 doi: 10.1007/s11684-008-0018-x

Abstract: To construct a polymerase chain reaction (PCR) site-directed mutagenesis of the long QT syndrome KCNQ1Mutagenesis was performed in a two-step PCR.and inserted into the same restriction site of pIRES-EGFP-KCNQ1.The sequencing analysis shows that the mutation site was correct.Mutation from A to G in site 983 of KCNQ1 cDNA was found.

Keywords： restriction     digestion     syndrome     sequence     site-directed mutagenesis    

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Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 66-71 doi: 10.1007/s11705-016-1591-1

Abstract: Cyanobacteria can produce useful renewable fuels and high-value chemicals using sunlight and atmospheric carbon dioxide by photosynthesis. Genetic manipulation has increased the variety of chemicals that cyanobacteria can produce. However, their uniquely abundant NADPH-pool, in other words insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme, which is quite common in heterotrophic microbes. To overcome this cofactor imbalance and enhance cyanobacterial fuel and chemical production, various approaches for cofactor engineering have been employed. In this review, we focus on three approaches: (1) utilization of NADPH-dependent enzymes, (2) increasing NADH production, and (3) changing cofactor specificity of NADH-dependent enzymes from NADH to NADPH.

Keywords： NADH-dependent enzyme     NADPH-dependent enzyme     transhydrogenase     site-directed mutagenesis     enzyme engineering    

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Rational Design, Over-expression and Characterization of a Novel Staphylokinase with Lower Tendency of Polymerization

Song Gang,Yu Min,Mo Wei,Song Houyan

Strategic Study of CAE 2000, Volume 2, Issue 11,   Pages 68-72

Abstract: RGD-Sak gene was constructed by substitution mutagenesis of Lys-109 and Phe-111 with Arg-109 and Asp

Keywords： staphylokinase     dimer     molecular docking     molecular design     site-directed mutagenesis    

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Efficient Activity Enhancement of a Lipase from Sporisorium reilianum for the Synthesis of a Moxifloxacin Chiral Intermediate via Rational Design Article

Xue Cai, Jiang-Wei Shen, Yu Qiang, Jing Hua, Zhang-Qi Ma, Zhi-Qiang Liu, Yu-Guo Zheng

Engineering 2022, Volume 19, Issue 12,   Pages 207-216 doi: 10.1016/j.eng.2022.03.020

Abstract: A tetra-site variant, SRL-I194N/V195L/L145V/L154G (V13), with a significantly enhanced activity of 87.8

Keywords： Lipase     Sporisorium reilianum     Site-directed mutagenesis     Molecular dynamics simulation     Rational    

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Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Frontiers of Medicine 2011, Volume 5, Issue 4,   Pages 356-371 doi: 10.1007/s11684-011-0159-1

Abstract: This review will summarize current information on the mechanisms of insertional mutagenesis in hematopoietic

Keywords： gene therapy     hematopoietic stem cells     insertional mutagenesis     genotoxicity     induced pluripotent stem cell    

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Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 519-525 doi: 10.1007/s11783-011-0326-2

Abstract: Application of directed evolution technology may be one of the most effective ways for improving thehydrolysis mechanism by cellulase, advances in cellulases (endoglucanase and -glucosidase) improvement by directedcharacteristics (for instance, thermal stability, pH adaptability and enzyme activity), limitations of directedevolution for cellulases, and the outlook for directed evolution for cellulase.

Keywords： biohydrogen     bioethanol     cellulase     cellulose     directed evolution    

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analysis of with enhanced poly--glutamic acid production through atmospheric and room temperature plasma mutagenesis

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 12,   Pages 1751-1760 doi: 10.1007/s11705-022-2211-x

Abstract: Poly-γ-glutamic acid is an extracellular polymeric substance with various applications owing to its valuable properties of biodegradability, flocculating activity, water solubility, and nontoxicity. However, the ability of natural strains to produce poly-γ-glutamic acid is low. Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacillus licheniformis CGMCC 2876, and a mutant strain M32 with an 11% increase in poly-γ-glutamic acid was obtained. Genome resequencing analysis identified 7 nonsynonymous mutations of ppsC encoding lipopeptide synthetase associated with poly-γ-glutamic acid metabolic pathways. From molecular docking, more binding sites and higher binding energy were speculated between the mutated plipastatin synthase subunit C and glutamate, which might contribute to the higher poly-γ-glutamic acid production. Moreover, the metabolic mechanism analysis revealed that the upregulated amino acids of M32 provided substrates for glutamate and promoted the conversion between L- and D-glutamate acids. In addition, the glycolytic pathway is enhanced, leading to a better capacity for using glucose. The maximum poly-γ-glutamic acid yield of 14.08 g·L–1 was finally reached with 30 g·L–1 glutamate.

Keywords： ARTP mutagenesis     Bacillus licheniformis     poly-γ-glutamic acid     metabolomics    

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Unnormalized and normalized forms of gefura measures in directed and undirected networks

Raf GUNS,Ronald ROUSSEAU

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 4,   Pages 311-320 doi: 10.1631/FITEE.1400425

Abstract: latter normalizes at the level of nodes. (2) Treating undirected networks as equivalent to symmetric directednetworks, we expand the definition of gefura measures to the directed case. (3) It is shown how Brandes

Keywords： Networks subdivided in groups     Partitions     Gefura measures     Q-measures     Brokerage role     Directed and    

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Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection

Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 10,   Pages 838-847 doi: 10.1631/FITEE.1500069

Abstract: Each agent is assumed to be governed by a second-order consensus control law corresponding to a directedThe first is concerned with directed trees, which represent the class of topology with minimum informationThe second is concerned with a more general graph that can be reduced to a directed tree by contracting

Keywords： Multi-agent systems     Controllability     Directed tree    

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Influence of site conditions on seismic design parameters for foundations as determined via nonlinearsite response analysis

Muhammad Tariq A. CHAUDHARY

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 1,   Pages 275-303 doi: 10.1007/s11709-021-0685-0

Abstract: Site conditions, including geotechnical properties and the geological setting, influence the near-surfaceparameters ( , , and ) was investigated for National Earthquake Hazards Reduction Program (NEHRP) siteclasses C and D, through 1D nonlinear seismic site response analysis.Predictive relationships for , , and were derived based on the 1D seismic site response analysis

Keywords： site effects     1D seismic site response analysis     sensitivity analysis     foundations     shear wave velocity     soil    

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Ionic strength directed self-assembled polyelectrolyte single-bilayer membrane for low-pressure nanofiltration

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 5,   Pages 699-708 doi: 10.1007/s11705-021-2093-3

Abstract: In this work, an ionic strength directed self-assembly procedure is described for the preparation of

Keywords： layer-by-layer self-assembly     single bilayer     nanofiltration membrane     desalination    

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Combination of ARTP mutagenesis and color-mediated high-throughput screening to enhance 1-naphthol yield

Chenggang Qiu, Alei Zhang, Sha Tao, Kang Li, Kequan Chen, Pingkai Ouyang

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5,   Pages 793-801 doi: 10.1007/s11705-019-1876-2

Abstract: Strain QCG of the aerobic bacteria is capable of producing 1-naphthol from naphthalene, this strain was first isolated and characterized in this study. Strain QCG was mutagenized to enhance 1-naphthol production, using atmospheric and room temperature plasma (ARTP) technology. Then, a microbial clone screening system was used to accelerate the operation. Meanwhile, a novel color-mediated high-throughput screening using 4-aminoantipyrine was performed to screen mutants. The optimal mutant strain QCG4 produced 19.58±0.34 mg∙L 1-naphthol from naphthalene that was 47.32% higher than that of the original strain (13.29±0.28 mg∙L ). In addition, the optimal conditions for 1-naphthol production via whole-cell catalysis of strain QCG4 were determined to be an OD of 40, 150 mg∙L naphthalene, and 7.5% dimethyl formamide as a co-solvent at pH 7.5 and 26°C for 3 h, resulting in 41.18±0.12 mg∙L 1-naphthol, i.e., the mutant strain produces a 2.1-fold higher yield compared to the original strain.

Keywords： Bacillus cereus QCG     naphthalene     1-naphthol     ARTP mutagenesis     high-throughput screening     4-aminoantipyrine    

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Mutagenesis and selective breeding of a high producing

Tian WANG, Shiru JIA, Zhilei TAN, Yujie DAI, Shuai SONG, Guoliang WANG

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2,   Pages 179-183 doi: 10.1007/s11705-012-1273-6

Abstract: -Poly- -lysine ( -PL) is an -lysine linear homopolymer, which is produced by bacteria belonging to the family and by ergot fungi. However, the production of -PL by the wild bacteria strain is very low, which limits its utilization. In most bacteria including the genus, -lysine is a precursor of -PL and is biosynthesized by the -aspartate pathway. Aspartokinase (Ask) is the first key enzyme in this pathway and is subject to complex regulation such as the feedback inhibition by the end product amino acids. In addition, phosphoenolpyruvate carboxykinase is feedback-regulated by -aspartate. To reduce these feedback inhibitions and to improve -PL productivity, resistant mutants were produced using sulfaguanidine (SG) + glycine+ -lysine+ -3-hydroxynorvaline (AHV) as selective markers. Using the interaction between -PL and the charged dye in the solid culture medium, hundreds of colonies were simultaneously screened in a quick and effective manner. Finally, one -PL-producing strain, L9, was selected. The productivity of this strain during flask fermentation was 0.77 g/L, which was 15% higher than that of the original strain. Moreover, its fermentation performance and genetic characteristics were very stable.

Keywords： ?-poly-L-lysine     plasma     AHV     Streptomyces diastatochromogenes     fermentation    

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Development and application of an off-site maintenance system in the petrochemical industry

Ruisheng YONG, Yanbing YE, Hanbin LUO, Lieyun DING

Frontiers of Engineering Management 2017, Volume 4, Issue 2,   Pages 111-126 doi: 10.15302/J-FEM-2017027

Abstract: On-site maintenance (ONSM) is critical to ensuring the safety of equipment in the petrochemical industryTo reduce congestion and exposure to hazards during on-site operations and thereby reduce the probabilityof accidents, off-site maintenance (OFSM) is proposed based on Energy Release Theory (ERT).

Keywords： on-site maintenance     petrochemical industry     safety     off-site maintenance system    

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Liver-directed treatment is associated with improved survival and increased response to immune checkpoint

Frontiers of Medicine   Pages 878-888 doi: 10.1007/s11684-023-0993-y

Abstract: Due to low response rates to systemic therapies, liver-directed therapies (LDT) are commonly used for

Keywords： uveal melanoma     liver-directed therapy     immune checkpoint blockade     SIRT     anti-PD-1     anti-CTLA-4    

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Title Author Date Type Operation

Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

Journal Article

Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Journal Article

Rational Design, Over-expression and Characterization of a Novel Staphylokinase with Lower Tendency of Polymerization

Song Gang,Yu Min,Mo Wei,Song Houyan

Journal Article

Efficient Activity Enhancement of a Lipase from Sporisorium reilianum for the Synthesis of a Moxifloxacin Chiral Intermediate via Rational Design

Xue Cai, Jiang-Wei Shen, Yu Qiang, Jing Hua, Zhang-Qi Ma, Zhi-Qiang Liu, Yu-Guo Zheng

Journal Article

Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Journal Article

Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

Journal Article

analysis of with enhanced poly--glutamic acid production through atmospheric and room temperature plasma mutagenesis

Journal Article

Unnormalized and normalized forms of gefura measures in directed and undirected networks

Raf GUNS,Ronald ROUSSEAU

Journal Article

Controllability analysis of second-ordermulti-agent systemswith directed andweighted interconnection

Di GUO,Rong-hao ZHENG,Zhi-yun LIN,Gang-feng YAN

Journal Article

Influence of site conditions on seismic design parameters for foundations as determined via nonlinearsite response analysis

Muhammad Tariq A. CHAUDHARY

Journal Article

Ionic strength directed self-assembled polyelectrolyte single-bilayer membrane for low-pressure nanofiltration

Journal Article

Combination of ARTP mutagenesis and color-mediated high-throughput screening to enhance 1-naphthol yield

Chenggang Qiu, Alei Zhang, Sha Tao, Kang Li, Kequan Chen, Pingkai Ouyang

Journal Article

Mutagenesis and selective breeding of a high producing

Tian WANG, Shiru JIA, Zhilei TAN, Yujie DAI, Shuai SONG, Guoliang WANG

Journal Article

Development and application of an off-site maintenance system in the petrochemical industry

Ruisheng YONG, Yanbing YE, Hanbin LUO, Lieyun DING

Journal Article

Liver-directed treatment is associated with improved survival and increased response to immune checkpoint

Journal Article